Fig. 5

Contribution of JNK activation on apoptosis induced by EEPK. MC3 and YD-15 cells were pretreated with the indicated doses of SP600125 for 1 h and then treated with DMSO or EEPK for 24 h. a Western blotting was performed to detect the expression levels of cleaved PARP. β-actin was used as an internal control. b Annexin V/PI double staining was measured by FACS analysis. c Representative images of DAPI-stained cells (magnification, 400×). The white arrows indicate apoptotic cells. Scale bar, 20 μm. All bar graphs represent the mean ± SD of three independent experiments. ^*p < 0.05; ^#p < 0.05