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Table 1 Composition of culture medium (CM) used for BC PDOs growth

From: Ultrastructural analysis of breast cancer patient-derived organoids

Reagents

Supplier

Catalogue number

Final Concentration

DMEM/F12

HyClone

D8437

1X

L-glutamine

Corning

25-005-CI

1%

Penicillin/Streptomicin

Corning

30-003_CI

1%

Hepes

Sigma

83264

10 mM

Noggin conditioned medium

Home made

25×

B 27 supplement

Gibco

17504-044

N-acetyl-cysteine

Sigma

A9165

1.25 mM

Nicotinamide

Sigma

N0636

0.2 mM

A 83-01

Tocris

2939

500 nM

Y-27632

ForLab

M1817

5 µM

R-spondin1 conditioned medium

Home made

10%

Primocin

Invivogen

ANT-PM-2

50 µg/mL

Human EGF

Peprotech

AF-100-15

5 ng/mL

FGF-10 HUMAN recombinant

Peprotech

100-26-25

20 ng/mL

KGF/FGF-7 Human recombinant

Peprotech

100-19-10

5 ng/mL

Heregulin-beta-1 Human recombinant

Peprotech

100-03-50

37.5 ng/mL

SB 202190

Sigma

S7067

500 nM