From: Ultrastructural analysis of breast cancer patient-derived organoids
Reagents | Supplier | Catalogue number | Final Concentration |
---|---|---|---|
DMEM/F12 | HyClone | D8437 | 1X |
L-glutamine | Corning | 25-005-CI | 1% |
Penicillin/Streptomicin | Corning | 30-003_CI | 1% |
Hepes | Sigma | 83264 | 10 mM |
Noggin conditioned medium | Home made | – | 25× |
B 27 supplement | Gibco | 17504-044 | 1× |
N-acetyl-cysteine | Sigma | A9165 | 1.25 mM |
Nicotinamide | Sigma | N0636 | 0.2 mM |
A 83-01 | Tocris | 2939 | 500 nM |
Y-27632 | ForLab | M1817 | 5 µM |
R-spondin1 conditioned medium | Home made | – | 10% |
Primocin | Invivogen | ANT-PM-2 | 50 µg/mL |
Human EGF | Peprotech | AF-100-15 | 5 ng/mL |
FGF-10 HUMAN recombinant | Peprotech | 100-26-25 | 20 ng/mL |
KGF/FGF-7 Human recombinant | Peprotech | 100-19-10 | 5 ng/mL |
Heregulin-beta-1 Human recombinant | Peprotech | 100-03-50 | 37.5 ng/mL |
SB 202190 | Sigma | S7067 | 500 nM |