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Table 1 Composition of culture medium (CM) used for BC PDOs growth

From: Ultrastructural analysis of breast cancer patient-derived organoids

Reagents Supplier Catalogue number Final Concentration
DMEM/F12 HyClone D8437 1X
L-glutamine Corning 25-005-CI 1%
Penicillin/Streptomicin Corning 30-003_CI 1%
Hepes Sigma 83264 10 mM
Noggin conditioned medium Home made 25×
B 27 supplement Gibco 17504-044
N-acetyl-cysteine Sigma A9165 1.25 mM
Nicotinamide Sigma N0636 0.2 mM
A 83-01 Tocris 2939 500 nM
Y-27632 ForLab M1817 5 µM
R-spondin1 conditioned medium Home made 10%
Primocin Invivogen ANT-PM-2 50 µg/mL
Human EGF Peprotech AF-100-15 5 ng/mL
FGF-10 HUMAN recombinant Peprotech 100-26-25 20 ng/mL
KGF/FGF-7 Human recombinant Peprotech 100-19-10 5 ng/mL
Heregulin-beta-1 Human recombinant Peprotech 100-03-50 37.5 ng/mL
SB 202190 Sigma S7067 500 nM