Fig. 7

Bufalin induces U251 cell apoptosis by regulating Annexin A2 and DRP1 protein expression. A The expression of Annexin A2 protein in U251 cytoplasm and mitochondria after bufalin treatment for 24 h (n = 3). B Statistical analysis of Annexin A2 in U251 cytoplasm and mitochondria after bufalin treatment. C Verification of the Annexin A2 siRNA silencing effect. D The viability of U251 cells upon Annexin A2 silencing and bufalin treatment (n = 6). E The protein expression levels of DRP1 and MFN2 in the cytoplasm and mitochondria of U251 cells treated with bufalin for 24 h (n = 3). F Statistical analysis of DRP1 and MFN2 in U251 cytoplasm and mitochondria after bufalin treatment. G Mitochondrial morphology and structure of U251 cells were observed with TEM (n = 3). H Viability of U251 cells pretreated with Mdivi-1 (n = 6). I Stereology analyses the Rsv, Vv, Sv and Nv of mitochondria after pretreatment with Mdivi-1. J The intracellular ATP content of U251 cells (n = 3). P values were determined using one-way ANOVA. The relative expression value for each sample is shown along with the mean ± SD for each group. *p < 0.05, **p < 0.01 compared with DMSO control