Fig. 8

NCAPG promotes lung adenocarcinoma progression and proliferation through the TGF-β signaling pathway. a GSEA analysis revealed that the abnormal expression of NCAPC is significantly related to the TGF-β signaling pathway. b Western blot analysis of p-Smad3, Smad3, p-Smad2, and Smad2. c Transwell assays were performed to investigate the role of the TGF-β signaling pathway in the migration and invasion ability of NCAPC-overexpressed A549 cells. d Clone formation assays were performed to investigate the role of the TGF-β signaling pathway in the proliferation ability of NCAPC-overexpressed A549 cells. e Western blot analysis of E-cadherin, N-cadherin, vimentin, and Snail was performed to determine the role of the TGF-β signaling pathway in the epithelial–mesenchymal transition of NCAPG-overexpressed A549 cells. f Western blot analysis of p-Smad3, Smad3, p-Smad2, and Smad2 was performed to determine the effect of RepSox after NCAPG overexpression. *p < 0.05, **p < 0.01, ***p < 0.001