Fig. 1

TFF1 attenuates H. pylori-induced activation of NF-κB and STAT3 in vitro. a–b) Western blot analysis of STAT3 and NF-κB in AGS cell lines stably expressing TFF1 or empty vector pcDNA infected with H. pylori J166 (a) or 7.13 (b) strains at different time points 1, 6 and 24 h. H. pylori increases of P-NF-κB-P65 (S536) and P-STAT3 (Y705) protein levels after 1 and 24 h of infection, respectively and reconstitution of TFF1 expression abolished this increase. β-ACTIN was used as a protein loading control, and TFF1 expression was confirmed using TFF1 specific antibody. The relative intensity ratio of p-STAT3 (y705)/β-Actin and p-NF-κB/β-Actin were calculated by the Image-Lab software from BioRad. The Western blot results are representative of three independent experiments. The results are expressed as mean ± SEM of at least three independent experiments using a two-tailed Student’s t-test