Fig. 2
From: NF-kB-dependent activation of STAT3 by H. pylori is suppressed by TFF1
Reconstitution of TFF1 suppresses H. pylori-mediated nuclear localization of NF-κB and STAT3. (a) In vitro immunofluorescence assay showing nuclear localization of p-NF-κB–p65 and p-STAT3 in uninfected and H. pylori (7.13 strain) infected AGS stable cells. After infection, AGS-pcDNA cells demonstrated an increase of NF-κB–p65 (Green) and STAT3 (Red) nuclear localization (arrows). However, after reconstitution of TFF1, the percentage of nuclear staining of both NF-κB and STAT3 was decreased (arrowheads). DAPI (blue) was used as a nuclear counterstain. Original magnification, × 400. b–c The bar graphs are showing the quantification of nuclear NF-κB (b) and STAT3 (c) staining in at least 200 counted cells, presented as percentage ± SEM