Fig. 4
From: NF-kB-dependent activation of STAT3 by H. pylori is suppressed by TFF1
TFF1 abolishes H. pylori-induced increase of NF-κB and STAT3 binding to its target gene IL6. a–b ChIP assay in AGS-pcDNA and AGS-TFF1 stable cells infected with H. pylori (7.13) for a period of 4 h for NF-κB binding (a) and 24 h for STAT3 binding (b), followed by quantitative real-time PCR with primers designed for STAT3 and NF-κB binding site on IL6 promoter regions. Control primers were designed 500 base pair upstream of STAT3 binding site on the IL6 promoter. These primers were used as negative control (NC). Results are presented as a percentage of input