Fig. 5
From: NF-kB-dependent activation of STAT3 by H. pylori is suppressed by TFF1
BAY and Iκ-B super repressor inhibit STAT3 activation. a The luciferase reporter assay using a STAT3-Luc (a) reporter plasmids. H. pylori infection of AGS-pcDNA cells significantly increased the luciferase activity, which was reduced after treatment with BAY (10 µM) or transfection with Iκ-B super-repressor plasmid The bar graphs represent the mean ± SEM of 3 independent experiments. b Western blot analysis of p-STAT3 in AGS-pcDNA cell lines infected with H. pylori, 7.13 treated with BAY, or transfected with Iκ-B super repressor (Iκ-BSR). The increase of p-STAT (Y705) protein level in H. pylori-infected cells was abolished after treatment with BAY or transfected with Iκ-BSR plasmid. The results are representative of three independent experiments