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Fig. 2 | Cancer Cell International

Fig. 2

From: LncRNA CTD-3252C9.4 modulates pancreatic cancer cell survival and apoptosis through regulating IFI6 transcription

Fig. 2

LncRNA CTD-3252C9.4 affects migration, invasion, proliferation and apoptosis of pancreatic cancer cells in vitro. A CTD-3252C9.4 overexpression and knockdown efficiencies tested by transfecting CTD-3252C9.4 plasmid and CTD-3252C9.4 siRNAs in Panc-1 and MIApaca-2 cells. B Migration of CTD-3252C9.4-overexpressing or CTD-3252C9.4- knocked down Panc-1 and MIApaca-2 cells examined by wound healing assays. Scale bar, 200 μm. Migration (C) and invasion (D) of CTD-3252C9.4-overexpressing or CTD-3252C9.4- knocked down Panc-1 and MIApaca-2 cells were examined by transwell assays. Scale bar, 100 μm. Proliferation of CTD-3252C9.4-overexpressing or CTD-3252C9.4- knocked down Panc-1 and MIApaca-2 cells was examined by CCK-8 assays (E), plate colony assays (F, Scale bar, 1 cm) and EdU assays (G, Scale bar, 200 μm). H Apoptosis of CTD-3252C9.4-overexpressing or CTD-3252C9.4- knocked down Panc-1 and MIApaca-2 cells was examined by Annexin V/ propidium iodide (PI) staining and flow cytometry assays. All data are shown as the mean ± SEM. *P < 0.05 and **P < 0.01 by two-tailed Student’s t-test

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