Fig. 3

LncRNA CTD-3252C9.4 promotes pancreatic cancer growth and metastasis in vivo. A CTD-3252C9.4 overexpression efficiency by transfecting pLVX-CTD-3252C9.4 in Panc-1 cells. B Flowchart of the in vivo xenograft experiments designed for examining tumor proliferation and metastasis. The tumor growth of mice subcutaneously implanted with the indicated Panc-1 cells. Tumor volume (C) and weight (E) were measured, and tumor size is shown in picture (D). Representative H&E staining (F), Ki-67 and cleaved caspase 3 immunohistochemical staining (G) of the tumor sections. Quantitative analysis of the fluorescence intensities (H) and representative BLI images (I) of mice intravenously injected with the indicated Panc-1 cells on days 3, 10, 20, and 30 after injection. The gross lesions (left) and representative H&E-stained sections (right) of liver (J) and lung (K) tissues isolated from the intravenously injected mice. Black arrows indicate the metastatic nodules. Scale bar, 100 μm. All data are shown as the mean ± SEM. *P < 0.05 and **P < 0.01 by two-tailed Student’s t-test