Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: Contribution of nuclear BCL10 expression to tumor progression and poor prognosis of advanced and/or metastatic pancreatic ductal adenocarcinoma by activating NF-κB-related signaling

Fig. 3

Inhibition of BCL10 downregulates cell cycle proteins expression and NF-κB activation in PDAC cell lines. a In total cell lysates, transfection with shBCL10 downregulated the expression of p-CDC2, p-CDC25C, Cyclin B1 in PANC-1 cell lines and the expression of Cyclin A, E, and D1, CDK2, and CDK4 in BxPC-3 compared to scrambled (Scr) group and control (Con) group. When compared to scrambled (Scr) group and control (Con) group, shBCL10 transfection upregulated the expression of p21 and p27 in both PANC-1 and BxPC-3 cells. b shBCL10 transfection decreased the expressions of BCL3, c-Myc, p-IκBα, and NF-κB (p65) compared to scrambled (Scr) group and control (Con) group in nuclear lysates of both PANC-1 and BxPC-3 cells. c The results from three independent experiments using shBCL10-transfected PANC-1 and BxPC-3 cells are presented as relative luciferase units (RLU) per milligram of protein (NF-κB-Luc promoter activity-luciferase assay) (**p < 0.01). d PANC-1 cells and BxPC-3 cells were treated with BAY117082 (2 µM) for 24 h and compared with vehicle (0.01% DMSO) treated groups. *p < 0.05; n = 3. e PANC-1 cells and BxPC-3 cells were treated as described in (d), the expressions of BCL10, BCL3, p-p65, and p65 in nuclear lysates of PANC-1 cells and BxPC-3 cells, and the expressions of Cyclin B1, Cyclin D1, and p21 in total lysates were determined by western blotting. β-actin was used as the loading control. PDAC pancreatic ductal adenocarcinoma

Back to article page