Fig. 5

Nb CAR-T cell cytotoxicity on patient-derived tumour cells. A Expression of CD19 and CD20 in patient samples. Patient PBMCs were harvested and stained with PE-conjugated mouse anti-human CD19 (BD) and APC-conjugated mouse anti-human CD20 (BD) at room temperature for 30 min, washed and resuspended in FACS buffer, Flow cytometry was used to assess CD19 and CD20 expression. B, C The proliferation and activation of Nb CAR-T cells stimulated by primary ALL tumour cells. Nb CAR-T cells and tumour cells were coincubated overnight or 5 days, followed by Flow cytometry analysis. D Nb CAR-T cell cytotoxicity to primary ALL tumour cells. Nb CAR-T cells were incubated with primary tumour cells, and LDH-based cytotoxicity assays were performed. E Cytokine production of Nb CAR-T cells to primary tumour cells. Nb CAR-T cells and primary tumour cells were coincubated overnight, and then, the expression of IL-2 was determined by ELISAs. Data were analysed by one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant (p > 0.05)