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Fig. 2 | Cancer Cell International

Fig. 2

From: Knockdown lncRNA CRNDE enhances temozolomide chemosensitivity by regulating autophagy in glioblastoma

Fig. 2

Knockdown CRNDE enhanced chemosensitivity to TMZ and reduced ABCG2 expression in vitro. a CCK-8 assays revealed cell viability with the exposure of TMZ depending on different doses for 72 h and IC50 values after si-NC or si-CRNDE transfection in three cell lines, respectively. *P < 0.05, **P < 0.01 and ***P < 0.001 represent si-CRNDE groups compared with si-NC groups, respectively. Colony formation assays (b) and EdU assays (c) showed cell proliferation ability of si-NC or si-CRNDE cells with or without exposure of TMZ at 100 μM for 72 h in three cell lines, respectively. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with si-NC cells without TMZ treatment, *#P < 0.05, **#P < 0.01 compared with si-NC group cells with TMZ treatment. d Cell apoptosis analysis revealed cell apoptosis ability of si-NC or si-CRNDE cells with or without exposure of TMZ at 100 μM for 72 h in three cell lines, respectively. *P < 0.05, **P < 0.01 compared with si-NC cells without TMZ treatment, *#P < 0.05, **#P < 0.01 compared with si-NC group cells with TMZ treatment. Scale bars = 100 μΜ. e Western blot detected the ABCG2 protein expression after si-NC or si-CRNDE transfection in three cell lines treated with TMZ at 100 μM for 72 h, respectively. Data represent mean ± SD from 3 independent experiments

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