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Fig. 4 | Cancer Cell International

Fig. 4

From: Knockdown lncRNA CRNDE enhances temozolomide chemosensitivity by regulating autophagy in glioblastoma

Fig. 4

CRNDE regulated TMZ-induced autophagy through PI3K/Akt/mTOR pathway in glioma cells. a Western blot revealed the protein levels of p-PI3K/PI3K, p-Akt/Akt and p-mTOR/mTOR in PI3K/Akt/mTOR pathway and LC3 II/I, Beclin 1 and p62 in autophagy after CRNDE knockdown and addition with LY294002 with exposure of TMZ at 100 μM for 72 h in PGC line. *P < 0.05, **P < 0.01. b CCK-8 assays the cell viability after CRNDE knockdown and addition with LY294002 with exposure of TMZ at 100 μM for different hours in PGC line. *P < 0.05, **P < 0.01 represent si-CRNDE groups were compared with si-NC groups, respectively. #P < 0.05, ##P < 0.01 represent si-CRNDE + LY294002 groups were compared with si-CRNDE groups, respectively. c EdU assays detected the cell proliferation after CRNDE knockdown and addition with LY294002 with exposure of TMZ at 100 μM for 72 h in PGC line. **P < 0.01 represent si-CRNDE group was compared with si-NC group. #P < 0.05 represent si-CRNDE + LY294002 groups was compared with si-CRNDE group. Scale bars = 100 μΜ. d Cell apoptosis analysis revealed cell apoptosis ability after CRNDE knockdown and addition with LY294002 with exposure of TMZ at 100 μM for 72 h in PGC line. *P < 0.05 represent si-CRNDE group was compared with si-NC group. #P < 0.05 represent si-CRNDE + LY294002 groups was compared with si-CRNDE group. Data represent mean ± SD from 3 independent experiments

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