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Fig. 2 | Cancer Cell International

Fig. 2

From: Circ-SFMBT2 drives the malignant phenotypes of esophageal cancer by the miR-107-dependent regulation of SLC1A5

Fig. 2

Silence of circ-SFMBT2 suppressed proliferation, invasion and glutamine metabolism in EC cells. Si-NC or si-circ-SFMBT2 was transfected into KYSE150 and TE1 cells. A The qRT-PCR was used for the quantification of circ-SFMBT2 expression after transfection for 48 h. B, C MTT assay was used for the analysis of cell proliferation after transfection for 48 h. D Colony formation assay was used for proliferation detection at 24 h post-transfection. E EdU assay was used to assess proliferation at 48 h post-transfection. F Flow cytometry was used for the examination of cell apoptosis after transfection for 72 h. G Transwell assay was applied to evaluate cell invasion after transfection for 24 h. HJ The glutamine consumption (H), α-ketoglutarate production (I) and glutamate production (J) by the corresponding kits were used for the assessment of glutamine metabolism after cell transfection for 48 h. (K-L) Western blot was used for the protein detection of CyclinD1 and MMP9 after cell transfection for 48 h. **P < 0.01, ***P < 0.001, ****P < 0.0001

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