Fig. 4

The si-circ-SFMBT2-mediated anti-tumor function was reversed by miR-107 inhibitor in EC cells. KYSE150 and TE1 cells were transfected with si-NC, si-circ-SFMBT2, si-circ-SFMBT2 + anti-miR-NC or si-circ-SFMBT2 + anti-miR-107. A The miR-107 expression was assayed using qRT-PCR. B–E Cell proliferation was examined using MTT assay (B–C), colony formation assay (D) and EdU assay (E). F, G Cell apoptosis (F) and invasion (G) were respectively detected using flow cytometry and transwell assay. H–J Glutamine metabolism was evaluated using the glutamine consumption (H), α-ketoglutarate production (I) and glutamate production (J) by the corresponding kits. K, L CyclinD1 and MMP9 protein levels were determined using western blot. **P < 0.01, ***P < 0.001, ****P < 0.0001