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Fig. 6 | Cancer Cell International

Fig. 6

From: Circ-SFMBT2 drives the malignant phenotypes of esophageal cancer by the miR-107-dependent regulation of SLC1A5

Fig. 6

SLC1A5 downregulation impeded the malignant behaviors of EC cells. Transfection of si-con or si-SLC1A5 was performed in KYSE150 and TE1 cells. A The expression analysis of SLC1A5 was conducted by western blot. B–E Cell proliferation detection was conducted by MTT assay (B, C), colony formation assay (D) and EdU assay (E). F, G The evaluation of cell apoptosis (F) and invasion (G) was conducted by flow cytometry and transwell assay. H–J The measurement of glutamine metabolism was performed by the glutamine consumption (H), α-ketoglutarate production (I) and glutamate production (J) using the detection kits. K, L The protein quantification of CyclinD1 and MMP9 was performed by western blot. ***P < 0.001, ****P < 0.0001

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Cancer Cell International

ISSN: 1475-2867

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