Fig. 4

miR-1180-3p negatively regulates melanoma cells’ growth, migration and invasion. A Interfere with the expression of miR-1180-3p in melanoma cells. RNAs were extracted from SK-Mel-28 infected with mimic and inhibitor of miR-1180-3p, and RT-Q-PCR was performed as described in Methods. Data from multiple experiments are expressed as the mean ± SD (n = 3). Significant differences were evaluated using Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001. B miR-1180-3p negatively regulates the growth of melanoma cells. SK-Mel-28 transfected with mimic or inhibitor of miR-1180-3p were seeded into 96-well plates, and cell viability was examined by CCK-8 kit as described in Methods. Data from multiple experiments are expressed as the means ± SD. Significant differences were evaluated using Two-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001. C, D miR-1180-3p regulates melanoma cell migration. The scratch assay was performed as described in Methods. Representative images were taken at indicated hours (C and D upper panel) and bar chart graphs shown are from three independent experiments (C and D lower panel). Data are presented as the mean ± SD (n = 3). Significant differences were evaluated using one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001. E–H miR-1180-3p regulates the invasion ability of melanoma cells. Transwell assays were performed as described in Methods. Representative images were taken at indicated hours (E and G). The number of invasive cells per field was calculated, and the data was presented as the means ± SD (n = 4) of each group (F and H). The significant difference between cells was evaluated by Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001