Fig. 5
From: The plasma exosomal miR-1180-3p serves as a novel potential diagnostic marker for cutaneous melanoma
MAN2B1 and ST3GAL4 might be target genes of miR-1180-3p and negatively regulated by miR-1180-3p. A Venn diagram of predicted target genes. Target genes predicted by miRWalk, ComiR and miRNA-Pathway were analyzed as described in Methods. B-L Validation of MAN2B1, MAN2C1, ST3GAL4 B3GALT2, FUT5, FUT6, FUT7, FUT9, MAN1C1, MAN2A2 and ST8SIA4 from the expression analysis in GEPIA. The box plots are based on 461 melanoma samples (red) and 558 normal samples (gray), revealing the overexpression of MAN2B1 (B) and ST3GAL4 (D) and the decreased expression of MAN2C1 (C) in melanoma. M miR-1180-3p negatively regulated the expression of MAN2B1 and ST3GAL4. RNA was extracted from melanoma cells transfected with mimic and inhibitor of miR-1180-3p. RT-Q-PCR was then performed with primers of MAN2B1 (left panel), MAN2C1 (middle panel) and ST3GAL4 (right panel) as described in the Methods. The data from multiple experiments (n = 3) are expressed as the mean ± SD. Significant differences were evaluated using one-way ANOVA, and an asterisk (*) indicates a significant difference (*p < 0.05, **p < 0.01, ***p < 0.001)