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Fig. 4 | Cancer Cell International

Fig. 4

From: The role of XBP-1-mediated unfolded protein response in colorectal cancer progression-a regulatory mechanism associated with lncRNA-miRNA-mRNA network

Fig. 4

KCNQ1OT1/XIST overexpression induced ER stress and suppressed cell apoptosis. A Ov-KCNQ1OT1 and Ov-NC were transfected into HCT116, and the expression level of KCNQ1OT1 was detected using qRT-PCR. B Ov-XIST and Ov-NC were transfected into HCT116, and the expression level of XIST was detected using qRT-PCR. C CCK-8 assay was conducted to analyze cell proliferation ability D After transfection, HCT116 cells were treated with or without 5-Fu, and the expression of GRP78 was detected using qRT-PCR. E TUNEL assay was conducted to determine cell apoptosis. F, G The protein expression of XBP-1 s, XBP-1u, Bcl-2, cleaved caspase3 and capase3 were detected using western blot. *, **, ***p < 0.05, 0.01 and 0.001 vs. Ov NC

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Cancer Cell International

ISSN: 1475-2867

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