Fig. 8

KCNQ1OT1/XIST overexpression aggravated tumor growth in vivo. A–C Mice were inoculated subcutaneously with HCT116 cells overexpressing KCNQ1OT1 or XIST. The tumor volume was recorded. After sacrifice, the tumor size was observed and tumor weight was recorded. D–F Mice were inoculated subcutaneously with HCT116 cells overexpressing KCNQ1OT1 or XIST and treated with 5-Fu. The tumor volume was recorded. After sacrifice, the tumor size was observed and tumor weight was recorded. G HE staining was performed to evaluate histological change, and IHC staining was performed to assess Ki67 and GRP78 expression in tumor. H, I western blot assay was performed to measure the protein expression of XBP-1s, XBP-1u, Bcl-2, cleaved caspase3 and caspase3. *, **, ***p < 0.05, 0.01, 0.001 vs Ov NC or 5-FU+Ov NC