Fig. 2

Functional study of miR-182-5p. a, d The relative expression of miR-182-5p was validated by qRT-PCR in cell lines transfected with the indicated oligonucleotides/plasmids. b, e Cell growth of the indicated cell lines in vitro was measured at different time points as indicated by CCK-8 assay. c, f The migratory abilities of the indicated cell lines were measured by transwell cell migration assay. Re-introduction of miR-182-5p promoted (c), whereas reduction of miR-182-5p inhibited (f) NPC cell migration. g, h HK1 cells stably infected with lentivirus containing Control-shRNA or miR-182-5p shRNA (182-shRNA) was inoculated subcutaneously into nude mice. The images (g) and weight (h) of xenograft tumors are shown. p = 0.0004, n = 6. i, j The effects of miR-182-5p on tumor metastasis were evaluated by the tail vein-lung metastasis model. i H&E staining of pulmonary sections from the tumor-bearing mice (scale bars = 50 μm). j Numbers of metastatic nodules per lung in the nude mice were summarized. p = 0.0189, n = 6. All data represent the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001, Student’s t-test