Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: The oncogenic role of HIF-1α/miR-182-5p/ZFP36L1 signaling pathway in nasopharyngeal carcinoma

Fig. 3

miR-182-5p inhibited ZFP36L1 expression by targeting its 3′ UTR in NPC cells. a The predicted duplex formation between the 3′ UTR of ZFP36L1 and miR-182-5p was shown. The wild-type (WT) and two mutants (MUT1 and MUT2) binding sites of the ZFP36L1 3′ UTR to miR-182-5p were cloned into the psiCHECK-2 vector. b, c Luciferase reporter assays were performed in HK1 (b) and CNE1 (c) cells co-transfected with Control mimic or miR-182-5p mimic together with the luciferase vectors containing the wide-type or mutant ZFP36L1 3′ UTR. **p < 0.01 and ***p < 0.001, Student’s t-test. d, e The expression of the indicated proteins was determined by Western blot analysis. ZFP36L1 was down-regulated by miR-182-5p mimic (d), but up-regulated by miR-182-5p inhibitor (e)

Back to article page