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Fig. 6 | Cancer Cell International

Fig. 6

From: The oncogenic role of HIF-1α/miR-182-5p/ZFP36L1 signaling pathway in nasopharyngeal carcinoma

Fig. 6

HIF-1α was involved in the up-regulation of miR-182-5p in NPC cells. a, b HK1 and CNE1 cells were exposed to 1% oxygen tension for 0 h, 24 or 48 h, and miR-182-5p levels were measured by qPCR. c Validation of cells expressing the indicated plasmids. HK1 and CNE1 cells overexpressing Vector or HIF-1α, as indicated, were subjected to western blot analysis. d The luciferase reporters containing a miR-182-5p promoter region was co-transfected with control Vector or HIF-1α into HK1 and CNE1 cells. Luciferase activities were detected after 48 h of transfection. e The relative expression of miR-182-5p was measured by qRT-PCR in cell lines overexpressing Vector or HIF-1α, as indicated. All data represent the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001, Student’s t-test

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