Fig. 2

LINC01605 is regulated by the SMYD2-EP300 complex. A staining of H3K4me3 and H3K27ac in cancer and ADJ tissues of CC patients examined using immunohistochemistry. B Spearman’s correlation analysis of the staining intensity of H3K27ac or H3K4me3 with the expression of LINC01605 in CC tissues. C H3K27ac or H3K4me3 recruitment levels of LINC01605 promoter in tumor and ADJ tissues of four CC patients measured using ChIP-qPCR. D R TFBS package and the JASPAR website prediction of regulatory factors that bind to the LINC01605 promoter. E expression of SMYD2 and EP300 in TCGA-COAD database; F SMYD2 or EP300 recruitment levels of LINC01605 promoter in tumor and ADJ tissues of four CC patients examined using ChIP-qPCR; G the subcellular localization of SMYD2 and EP300 in CC tissues detected using fluorescence co-localization assay. Error bars represent means ± SD for three independent experiments (**p < 0.01, two-way ANOVA, followed by Tukey’s multiple comparison)