Fig. 8From: LINC01605, regulated by the EP300-SMYD2 complex, potentiates the binding between METTL3 and SPTBN2 in colorectal cancerSPTBN2 overexpression reverses the repressive effects of LINC01605 silencing on CC cells. Overexpression plasmid of SPTBN2 was transfected into cells with stable low expression of LINC01605. A SPTBN2 protein expression in cells after co-transfection by Western blot. B the number of colonies formed by LoVo and Caco-2 examined using colony formation assay. C the proliferative activity of LoVo and Caco-2 examined using CCK-8 assay. D the proportion of apoptotic cells in LoVo and Caco-2 cells by flow cytometry. E the number of apoptosis bodies in cells by TUNEL assay. F, G the altered migration and invasion ability of LoVo and Caco-2 cells by Transwell assay. H the number of tubes formed by HUVEC cells co-cultured with Caco-2 and LoVo cells by tube formation assay. Error bars represent means ± SD for three independent experiments (**p < 0.01, two-way ANOVA, followed by Tukey’s multiple comparison)Back to article page