Fig. 2

Effect of Nestin knockdown on GC cell viability and apoptosis. GC cells were transfected with shRNA-NC and shRNA-Nestin-1, -2, and -3 for 36 h. A, B qPCR and C, D WB were performed to determine Nestin mRNA and protein expression level in GC cells, respectively. E, F The CCK-8 assay showed the viability of GC cells 2 d after transfection. G, H The bromodeoxyuridine (BrdU) assay showed the viability of GC cells 2 d after transfection. Scale bar, 50 μm. I, J Annexin V-FITC/PI FC showed the percentage of apoptotic cells 2 d after transfection (n = 3). The results are expressed as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the indicated groups