Fig. 4From: Nestin is essential for cellular redox homeostasis and gastric cancer metastasis through the mediation of the Keap1–Nrf2 axisEffect of Nestin knockdown on GC cell migration and invasion abilities. GC cells were transfected with shRNA-NC and shRNA-Nestin for 36 h. A The migration and B invasion abilities of GC cells were detected using wound healing assays and Transwell invasion assay. Scale bar, 50 μm. C qPCR and D, E WB analysis were used to determine the Bach1 mRNA and protein expression levels in GC cells, respectively, (n = 3). The results are expressed as means ± SD. **P < 0.01 vs. the indicated groupsBack to article page