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Fig. 5 | Cancer Cell International

Fig. 5

From: Nestin is essential for cellular redox homeostasis and gastric cancer metastasis through the mediation of the Keap1–Nrf2 axis

Fig. 5

Nestin protects Nrf2 expression in GC cells. GC cells were transfected with shRNA-NC and shRNA-Nestin for 36 h. AC qPCR revealed the effect of Nestin knockdown on the mRNA expression levels of Keap1, Nrf2, and HO-1 in GC cells. D, E WB analysis revealed the effect of Nestin knockdown on the protein expression levels of Keap1, Nrf2, and HO-1 in GC cells. F Nestin directly binds with Keap1. Flag-Nestin and myc-Keap1 were transfected into GC cells. Whole-cell lysates were used for immunoprecipitation using anti-Flag antibodies, and the proteins obtained were blotted with the indicator antibodies. G GC cells with Nestin knockdown were exposed to ARE luciferase reporter. The luciferase activity was determined 36 h after transfection (n = 3). The results are expressed as means ± SD. **P < 0.01 vs. the indicated groups

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