Fig. 6From: Nestin is essential for cellular redox homeostasis and gastric cancer metastasis through the mediation of the Keap1–Nrf2 axisNrf2 overexpression and SF treatment in GC cells. GC cells with Nestin knockdown were transfected with Nrf2-overexpressing vector for 36 h or treated with 10 μM SF for 36 h. A, B qPCR revealed the effect of Nestin knockdown on the mRNA expression levels of Nrf2 and HO-1 in GC cells. C WB revealed the effect of Nestin knockdown on the protein expression levels of Nrf2 and HO-1 in GC cells. D GC cells with Nestin knockdown were transfected with ARE luciferase reporter. The luciferase activity was determined 1.5 d after transfection. E–G qPCR revealed the effect of Nestin knockdown on the expression levels of the antioxidant-related genes GCLM, HMOX1, and NQO1 in GC cells (n = 3). The results are expressed as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the indicated groupsBack to article page