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Fig. 6 | Cancer Cell International

Fig. 6

From: Nestin is essential for cellular redox homeostasis and gastric cancer metastasis through the mediation of the Keap1–Nrf2 axis

Fig. 6

Nrf2 overexpression and SF treatment in GC cells. GC cells with Nestin knockdown were transfected with Nrf2-overexpressing vector for 36 h or treated with 10 μM SF for 36 h. A, B qPCR revealed the effect of Nestin knockdown on the mRNA expression levels of Nrf2 and HO-1 in GC cells. C WB revealed the effect of Nestin knockdown on the protein expression levels of Nrf2 and HO-1 in GC cells. D GC cells with Nestin knockdown were transfected with ARE luciferase reporter. The luciferase activity was determined 1.5 d after transfection. EG qPCR revealed the effect of Nestin knockdown on the expression levels of the antioxidant-related genes GCLM, HMOX1, and NQO1 in GC cells (n = 3). The results are expressed as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the indicated groups

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