Fig. 7From: Nestin is essential for cellular redox homeostasis and gastric cancer metastasis through the mediation of the Keap1–Nrf2 axisEffect of Nrf2 overexpression and SF treatment on cell viability and apoptosis in Nestin-depleted GC cells. GC cells with Nestin knockdown were transfected with Nrf2-overexpressing vector for 36 h or treated with 10 μM SF for 36 h. A The CCK-8 assay revealed the viability of GC cells 2 d after transfection. B Annexin V-FITC/PI FC revealed the percentage of apoptotic cells (n = 3). The results are expressed as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the indicated groupsBack to article page