Fig. 9

Effect of Nrf2 overexpression and SF treatment on the migration and invasion abilities of Nestin-knockdown GC cells. GC cells with Nestin knockdown were transfected with Nrf2-overexpressing vector for 36 h or treated with 10 μM SF for 36 h. A The migration and B invasion abilities of GC cells were assessed using wound healing and Transwell invasion assays. Scale bar, 50 μm. C qPCR and D WB analysis were used to determine the mRNA and protein expression levels of Bach1, respectively, in GC cells (n = 3). The results are expressed as means ± SD. *P < 0.05, **P < 0.01 vs. the indicated groups