Fig. 5
From: Mechanisms involved in the anti-tumor effects of Toosendanin in glioma cells
TSN induced cell viability, migration, invasion, and apoptosis by inhibiting the PI3K/Akt/mTOR signaling pathway. a Western blotting assay was performed to measure the expression levels of phosphorylated and non-phosphorylated PI3K, Akt, and mTOR proteins in U87MG and LN18 cells. b Then, the same cells were treated with 15 μM 740Y-P alone or 100 μM TSN alone or combined with 15 μM 740Y-P for 48 h; the same assay was performed to detect the protein levels of p-PI3K, PI3K, p-Akt, Akt, p-mTOR, and mTOR, MMP-2, cyclin D1, and Bcl-2