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Fig. 5 | Cancer Cell International

Fig. 5

From: Long noncoding RNA LINC00958 suppresses apoptosis and radiosensitivity of colorectal cancer through targeting miR-422a

Fig. 5

miR-422a suppresses cell proliferation, increases cell apoptosis and radiosensitization of colorectal cancer through MAPK1. a Bioinformatics databases predicted that MAPK1 was a target of miR-422a. b, c mRNA and protein expression of MAPK1 after knockdown or overexpression of miR-422a in colorectal cancer cells. d miR-422a mimics significantly decreased the luciferase activity of wild type of MAPK1, but not the activity of mutation of MAPK1. In addition, miR-422a inhibitor evidently increased the luciferase activity of wild type of MAPK1, but not it of mutation of MAPK1. e, i The flow cytometry assay showed that miR-422a mimics significantly increased the apoptosis percentage (e), while miR-422a inhibitor remarkably decreased the apoptosis percentage (i). f, g) and j, k The CCK-8 and MTT results indicated that miR-422a mimics decreased the ability of cell proliferation, and miR-422a inhibitor increased it. h, l miR-422a mimics enhanced the radiosensitization of colorectal cancer cell (h), while miR-422a inhibitor decreased the radiosensitization (l). Data are reported as means ± standard deviation of three independent experiments. **p < 0.01

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