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Fig. 5 | Cancer Cell International

Fig. 5

From: LncRNA MIR99AHG mediated by FOXA1 modulates NOTCH2/Notch signaling pathway to accelerate pancreatic cancer through sponging miR-3129-5p and recruiting ELAVL1

Fig. 5

NOTCH2 is targeted by miR-3129-5p. A RT-qPCR was applied for measuring NOTCH2 expression after transfection of miR-3129-5p mimics. B The binding region between miR-3129-5p and NOTCH2 was displayed. C The relationship among MIR99AHG, miR-3129-5p and NOTCH2 was examined by RIP assay. D Luciferase reporter assay was done for the detection of the luciferase activity in the NOTCH2 3’UTR-Wt group and NOTCH2 3’UTR-Mut group after transfection of miR-3129-5p mimics. E MiR-3129-5p inhibitor was transfected to cut down miR-3129-5p expression. F, G The detection of NOTCH2 expression was done by means of RT-qPCR and western blot in cells after transfection with indicated plasmids, including sh-NC, sh-MIR99AHG#1, sh-MIR99AHG#1 + NC inhibitor and sh-MIR99AHG#1 + miR-3129-5p inhibitor. **P < 0.01

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Cancer Cell International

ISSN: 1475-2867

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