Fig. 3

Knockdown of PGM1 attenuates GC viability and proliferation under glucose deprivation. a WB analysis to show the PGM1 expression in BGC-823 and MKN-28 cells stably carrying PGM1 shRNA or scrambled shRNA and additional transfection of PGM1 overexpression plasmids or vectors, respectively. b, c CCK-8 assay to measure viability of BGC-823 (b) and MKN-28 cells (c) as in (a) under the NG condition. d, e CCK-8 assay to measure viability of BGC-823 (d) and MKN-28 cells (e) as in (a) under the LG condition. f EdU assay to measure proliferation of cells as in (b, c). g EdU assay to measure proliferation of cells as in (d, e). Histograms are on the right. Scale bar: 100 μm. Data are shown as the mean ± SD. *P < 0.05, **P < 0.01, n.s. no significant. PGM1: Phosphoglucomutase 1; NG: normal glucose, 10 mM glucose in medium; LG: low glucose, 2.5 mM glucose in medium; NC: negative control; WB: western blot; CCK-8: cell counting kit-8; EdU: 5-ethynyl-2-deoxyuridine; DAPI: 4′,6-diamidino-2-phenylindole; SD: standard deviation