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Fig.5 | Cancer Cell International

Fig.5

From: Knockdown of PGM1 enhances anticancer effects of orlistat in gastric cancer under glucose deprivation

Fig.5

Orlistat inhibits lipid metabolism but promotes glycolysis in GC. a WB assay to show protein expression levels of BGC-823 and MKN-28 cells after treatment of DMSO, 20 μM, 40 μM or 60 μM orlistat for 24 h under the NG condition. b Colorimetry assay to measure FASN activity of cells as in (a). cf Colorimetry assays to measure glycolysis in GC cells after treatment of DMSO or 60 μM orlistat for 24 h under the NG condition, including lactate acid (c), pyruvic acid (d), ATP (e) production and glucose uptake capability (f). Data are shown as the mean ± SE. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. no significant. PGM1: Phosphoglucomutase 1; FASN: Fatty Acid Synthase; CPT1A: Carnitine Palmitoyltransferase 1A; ACC: Acetyl CoA Carboxylase; NG: normal glucose, 10 mM glucose in medium; NC: negative control; DMSO: dimethyl sulphoxide; SD: standard deviation

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