Fig.7

The combination of orlistat and PGM1 expression downregulation suppresses tumor growth and metastasis in vivo. a Representative bioluminescence images at 30 days after subcutaneous injection of luc-BGC-823 cells with knockdown of PGM1 or NC and treated with 240 μg/g of orlistat or the corresponding solvent daily. 3 mice in each group were displayed. b Curves of tumor volumes as in (a) at the indicated time. c Luminescence signals in (a) represented by overlaid false-color images with the signal intensity. d Lactate production was determined in the tumor tissues from (a). e FASN activity was determined in the tumor tissues from (a). f Representative bioluminescence images at 30 days after tail vein injection of luc-BGC-823 cells with knockdown of PGM1 or NC and treated with 240 μg/g of orlistat or the corresponding solvent daily. 3 mice in each group were displayed. g Luminescence signals in (f) represented by overlaid false-color images with the signal intensity. h Schematic illustration of mechanisms of PGM1 knockdown plus orlistat treatment in GC therapy. Data are shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. GC: gastric cancer; PGM1: Phosphoglucomutase 1; FASN: Fatty Acid Synthase; NC: negative control; SD: standard deviation