Fig. 3
m6A RNA methylation mediates LBX2-AS1 increased in CRC. A qRT-PCR analysis of LBX2-AS1 levels in the indicated CRC cell lines. B Identification of the specific m6A methylation loci of LBX2-AS1 using the SRAMP website. C m6A levels in LBX2-AS1 were measured by m6A-qPCR in CRC cells transfected with control (sh-NC) or METTL3 targeting (sh-METTL3) shRNAs. D qRT-PCR analysis of LBX2-AS1 levels relative to ACTIN in CRC cells treated with actinomycin D (10 μM). E qRT-PCR analysis of the level of LBX2-AS1 in the indicated CRC cell lines. F RIP assay carried out using anti-IGF2BP1 antibodies. For A, C, D, E and F, mean ± s.e.m. were derived from n = 3 independent experiments *P < 0.05; #P > 0.05; Student’s t-test