Fig. 6

LBX2-AS1 increased AKT1 level by sponging miR-422a in CRC. A Pearson correlation analysis using the online tool GEPIA indicated a strong positive correlation between the expression of AKT1 and LBX2-AS1 in CRC samples. B AKT1 mRNA was quantitated by RT-qPCR in HCT116 and SW480 cells after transfection with si-LBX2-AS1 or si-NC. C Bioinformatics analysis revealed the binding sites of miR-422a within LBX2-AS1. D miR-422a expression analysis performed using RT-qPCR. E Luciferase reporter assays were performed in indicated CRC cell lines. F LBX2-AS1 and miR-422a was enriched in Ago2-immunoprecipitates compared with the IgG control. G RT-qPCR detection of miR-422a expression in HCT116 and SW480 cells after transfection with antagomir-422a or antagomir-NC. H AKT1 mRNA levels measured by RT-qPCR in the indicated CRC cell lines. I P-gp, MRP1, and BCRP mRNA levels measured using RT-qPCR in the indicated CRC cell lines. For B, D, E–I, mean ± s.e.m. were derived from n = 3 independent experiments, *P < 0.05; **P < 0.01; ***P < 0.001; ^#P > 0.05; Student’s t-test