Fig. 2

Downstream effector analysis of differentially expressed genes in TNBC cells treated with DNMT inhibitors. a Tree map (hierarchical heat map) depicting affected functional categories based on differentially expressed genes in TNBC cells treated with DNMT inhibitors based on RNA-Seq and IPA analysis where the major colored boxes represent a category of diseases and functions where blue indicated decreasing while orange indicate increasing activation Z score. b Illustration of cell death and survival functional category. c Illustration of NUPR1, TP53, and NFKB mechanistic networks. The expression of selected number of differentially expressed genes was validated using qRT-PCR in MDA-MB-231 d and BT-549. e Data are presented as mean ± SEM (n = 6). *P < 0.05, **P < 0.005, ***P < 0.0005