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Fig. 4 | Cancer Cell International

Fig. 4

From: Transcriptional alterations of protein coding and noncoding RNAs in triple negative breast cancer in response to DNA methyltransferases inhibition

Fig. 4

Functional consequences of DNMTi on TNBC cells. MDA-MB-231 (a, b) and BT-549 (c, d) cells were treated with Decitabine and 5-Azacytidine at 2.0 µM. Cells were stained with AO/EO to detect apoptotic (cells with green condensed chromatin) and dead (red) cells (a, c). CFU assay was used to assess colony formation potential of MDA-MB-231 and BT-549 treated with DNMTi. Plates were stained with crystal violet on day 5. Data are representative of two independent experiments for each condition. Cell cycle analysis demonstrating the effects of DNMT inhibitors on cell cycle distribution in TNBC models (b, d). Quantification of cell cycle distribution is presented as bar diagram (n = 3). e CFU and qRT-PCR for TPX2 expression of MDA-MB-231 and BT-549 after treatment with siRNA targeting TPX2 or control siRNA on day 7. Quantification of CFU staining is presented on the right panel

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