Fig. 1

EMT-induced exosome characteristics of CRC cells.  A Representative image of the morphology of SW480 cells after TGF-β-induced EMT. The expression levels of E-cadherin (B) and VIM (C) in SW480 cells treated with TGF-β were detected by qRT-PCR. D Production of IFN-γ from NK92-MI cells when cultured alone or co-cultured with SW480 cell. E Transmission electron microscopy of exosomes derived from blank or TGF-β-induced SW480 cells. Scale bar, 100 nm. F The size and distribution of particles were detected by nanoparticle-tracking analysis. G The expression of the CD63 and CD9 biomarker in exosomes derived from SW480 cells was determined by western blotting. H Expression of TGF-β in SW480 cells induced by TGF-β or not. I Expression of TGF-β in exosomes derived from SW480 cells induced with TGF-β or not. GAPDH was used to normalize gene expression. t-test, *P < 0.05, **P < 0.01