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Fig. 3 | Cancer Cell International

Fig. 3

From: Licorice extract inhibits growth of non-small cell lung cancer by down-regulating CDK4-Cyclin D1 complex and increasing CD8+ T cell infiltration

Fig. 3

Licorice induces tumor cell G0/G1 phase arrested with the degradation of CDK4-Cyclin D1 complex. a H1975 cells were treated with different concentrations of licorice for 48 h, cell viability was determined using the CCK-8 assay. (mean ± SD, n = 6). b The cell-cycle profiles of H1975 cells incubated with 400, 600, 800 µg/ml GUF or vehicle control for 48 h were shown by using fluorescence-activated cell sorting (FACS). c Percentages of H1975 cells in b at different cell cycle states. d The protein expression in H1975 cells pretreated with 400,  600, 800 µg/ml GUF or vehicle control were measured by western blot anaylsis , versus β-actin as a loading control. e Relative protein abundance of CDK4 and Cyclin D1 of d. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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