Fig. 5
Licorice inhibits the growth of tumor volume depending on the CDK4-Cyclin D1 axis. a C57BL/6 mice were injected with 5×105 LLC cells. 24 h later, 200 mg/kg GUF or vehicle were administered once daily from day 2 and/or 200 μg/mouse anti-PD-L1 (i.p.) on day 4, 7, 10 (n = 5 per group). The tumor growth curve is shown, with tumor sizes presented as mean ± SD. b Primary tumor mass of mice is shown, presented as mean ± SD. c Protein expression in tumors from GUF group and control group was measured by western blot, versus β-actin as a loading control. d Relative protein abundance of PD-L1, CDK4, Cyclin D1, Cyclin B1 of c, *p < 0.05, **p < 0.01, ***p < 0.001, n.s.: no significant