Fig. 4

KIF2C overexpression reverses miR-152-induced DOX sensitivity in MCF7/ADR cells. A Levels of KIF2C in MCF7/ADR cells which were co-transfected with miR-NC, miR-152 mimics, or KIF2C. The data are presented as the means±SEM from three independent experiments. * Compared to miR-NC group at P < 0.05, ^## compared to miR-152 at P  <  0.01. B IC50 values of MCF7/ADR cells that were transfected as in A and were treated with DOX and cultured for 48 h. C Flow cytometric analysis of MCF7/ADR cells that were transfected as in (A) and were treated with 1 µg/ml DOX. D, E The glucose consumption (D, left), LA production (D, right), ECAR) (E, left) and OCR (E, right) were analyzed in MCF7/ADR cells treated as in A. F Levels of KIF2C, p62 and LC3 in MCF7/ADR cells treated as in (A). Graph represents the relative density of KIF2C, p62, or LC3-II/LC3-I expression. The data are presented as the means ± SEM from three independent experiments. ** Compared to miR-NC group at P < 0.01, ^## compared to miR-152 at P < 0.01. G The glucose consumption (G, top) and LA production (G, bottom) were analyzed in MCF7/ADR cells treated with TBX15 or KIF2C overexpression. H Flow cytometric analysis of MCF7/ADR cells that were transfected as in G and were treated with 1 µg/ml DOX. I Levels of p62 and LC3 in MCF7/ADR cells treated as in G. Graph represents the relative density of KIF2C, p62, or LC3-II/LC3-I expression. The data are presented as the means ± SEM from three independent experiments. *, ** Compared to control groups at P < 0.05 and P < 0.01, ^# compared to TBX15 at P  < 0.05