Fig. 4

UBTF binds the promoter region of GIT1 in melanoma cell. a Bioinformatics analysis (UCSC Genome Browser) predicted the UBTF binding site in the promoter of GIT1. b ChIP-QPCR showed that UBTF bound to the promoter region of GIT1 in A375/SK-MEL-28 cells. c ChIP-RT-PCR revealed that UBTF bound to the promoter region (Primer 6) of GIT1. d TCGA data showed that UBTF expression was positively correlated with GIT1 expression in human melanoma tissues. e qRT-PCR assay revealed the positive correlation between UBTF and GIT1 expressions. f A375/SK-MEL-28 cells were transfected with pGL3-GIT1-luc (target sequences of UBTF), the luciferase activity was examined at 48 h after transfection. Renilla luciferase was known as the internal control. g A375/SK-MEL-28 cells were co-transfected with pGL3-GIT1-luc and UBTF siRNAs; the luciferase activity was determined. h A375/SK-MEL-28 cells were co-transfected with pGL3-GIT1-luc and UBTF overexpression vector; the luciferase activity was measured. i UBTF siRNAs reduced GIT1 mRNA expression in melanoma cells. j UBTF overexpression vector enhanced GIT1 mRNA expression in melanoma cells. k UBTF siRNAs restrained GIT1 protein expression in melanoma cells. l UBTF overexpression vector promoted GIT1 protein expression in melanoma cells. *p < 0.01, n = 3