Fig. 6

GIT1 accelerates melanoma cell growth and inhibits apoptosis in vitro. a GIT1 mRNA expression was examined in A375/SK-MEL-28 cells after silencing GIT1. b GIT1 mRNA expression was measured after overexpressing GIT1. c MTT results showed melanoma cell activity after silencing GIT1. d MTT results revealed melanoma cell activity after overexpressing GIT1. e Cell colonies were measured 11 days after silencing GIT1. f Cell colonies were examined 11 days after overexpressing GIT1. g Cell cycle was analyzed. The histograms showed the proportion of G1/G0, S and G2/M phase cells after t silencing GIT1. h The histograms showed the percentage of G1/G0, S and G2/M phase cells after overexpressing GIT1. i The data represented the percentage of apoptosis after silencing GIT1. j The data revealed the ratios of apoptosis after overexpressing GIT1. k GIT1-MEK1/2-ERK1/2 signalling pathways were measured after silencing GIT1. l GIT1-MEK1/2-ERK1/2 signalling pathways were examined after overexpressing GIT1. *p < 0.01, n = 3