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Fig. 5 | Cancer Cell International

Fig. 5

From: Analyzing the whole-transcriptome profiles of ncRNAs and predicting the competing endogenous RNA networks in cervical cancer cell lines with cisplatin resistance

Fig. 5

AC010198.2/hsa-miR-34b-3p/STC2 pathway was verified by RNA pull-down and dual-luciferase reporter gene assays. (A–G) The RNA expression levels of AC010198.2 (A, E), miR-34b-3p (B, F) and STC2 (C, G) in CC cells and tissues (n = 7) were detected by RT-qPCR. D The protein level of STC2 in CC cells treated with si-AC010198.2 were detected by Western Blot. H–I) The highly expressed miR-34b-3p indicated a longer survival time in CESC, and the opposite association with STC2 was observed. J RT-qPCR after RNA pull-down assay showed that, in comparison to the miR-34b-3p-Mut-Bio probe, the use of biotin-labeled miR-34b-3p (miR-34b-3p-bio) probe can enrich AC010198.2 and STC2 RNA transcripts. K The luciferase activity analysis shows that miR-34b-3p can bind to the 3′UTR of STC2. L The luciferase activity analysis shows that miR-34b-3p can bind to AC010198.2. M Potential binding sites of STC2' 3'UTR and miR-34b-3p N Potential binding sites of miR-34b-3p and AC010198.2

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Cancer Cell International

ISSN: 1475-2867

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