Fig. 5
From: EF24 exerts cytotoxicity against NSCLC via inducing ROS accumulation
EF24 induces ROS accumulation, mitochondrial morphological changes and autophagy. A–B EF24 induces ROS accumulation. C EF24 induced mitochondrial fragmentation of the mitochondrial network in A549 cells. The arrow indicates fragmented mitochondria. D Western blot analysis demonstrated SQSTM1, LC3B and ACTB expression in EF24-treated A549 cells. E Autophagic vacuoles in A549 cells treated with various concentrations of EF24 were observed by TEM. The arrow indicates autophagic vacuoles. Number of autophagic vacuoles were calculated. F Changes in the localization of LC3B in cells assessed by immunofluorescence under confocal laser microscopy. The results are presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.01